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WebMar 30, 2024 · JEG-3 cells were counted and seeded at a density of 80,000 cells per well in black-walled, clear bottom 96-well plates (Corning #CLS3603, Millipore Sigma) and allowed to adhere and grow for 36 h. After approximately 36 h, 96-well plates were imaged on the IncuCyte Zoom to obtain cellular confluence measurements prior to chemical dosing (0 h). Web• 96 well plates (Corning CLS3603) • Box with ice Chemicals • Advanced DMEM/F-12 (Gibco-Thermo Fisher #12634028) • Glutamax 100x (GIBCO #35050038) • HEPES (1M) (GIBCO #15630080) • Pen/Strep 100x (GIBCO #15070063) • DPBS, 1x no calcium, no magnesium (Gibco #14190-094) • Sterile water • Fetal Bovine Serum (Biowest #S1810-500) locking lid garbage can
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