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WebGo to your Research tab. Select the research item you would like to generate a DOI for by clicking on its title. On the right-hand side, click on the More button and select Generate a DOI (if this is not visible, then generating a DOI for this type of research item is not possible) Review the details of your research item to ensure they are ... WebSep 4, 2014 · gRNA design and plasmid construction. Bicistronic expression vector (pX330) expressing both Cas9 and gRNA was generously provided by Dr. Feng Zhang of Broad Institute of MIT and Harvard . gRNAs targeting goat MSTN, NUP, PrP and BLG genes (Figure 1A) were designed as previously described .An extra guanine was added at the 5′ end of … WebJan 12, 2024 · ResearchGate. Instead of an institutional homepage, some authors self-archive their articles on ResearchGate. In the case of our paper, the full-text version is indeed directly available. And otherwise, if one of the authors is active on ResearchGate, it’s also possible to send a full-text request at the click of a button. Pirated version off ... mods for sims 4 youtube